Journal: Environmental Health Perspectives

Article Title: Seasonal Variations in Air Pollution Particle-Induced Inflammatory Mediator Release and Oxidative Stress

doi: 10.1289/ehp.7996

Figure Lengend Snippet: Production of ( A ) IL-6 by AM and ( B ) IL-8 by NHBE cells stimulated with coarse, fine, and ultrafine Chapel Hill pollution particles collected in four different months. The control IL-8 and IL-6 concentrations were 1.7 ± 0.2 ng/mL and 198 ± 5 pg/mL, respectively. Particles were added to NHBE cells at 11 μg/mL and to AMs at 50 μg/mL. *Tukey adjusted p -value < 0.05; n = 3–4 each. The dashed line denotes 1.0 (no change over control). The brackets indicate the groups that are significantly different by ANOVA and the Tukey subtest.

Article Snippet: Typically at confluency, NHBE cells in the 12-well plate (catalog no. 3512; Costar, Corning, NY) had a density of 300,000–500,000 cells/well in 2 mL of BEGM.

Techniques:

Journal: Environmental Health Perspectives

Article Title: Seasonal Variations in Air Pollution Particle-Induced Inflammatory Mediator Release and Oxidative Stress

doi: 10.1289/ehp.7996

Figure Lengend Snippet: Production of ROSs measured by DCF in ( A ) AM and ( B ) NHBE cells stimulated with coarse, fine, and ultrafine Chapel Hill pollution particles collected in four different months. Particles were added to NHBE cells at 11 μg/mL and to AMs at 50 μg/mL. In general, NHBE cells appeared to be more responsive to PM than the AMs because increases in DCF signals were similar between the two cell types even though NHBE cells were exposed to lower doses of PM. *Tukey adjusted p -value < 0.05; # p < 0.05 versus control (ratio = 1.0) by the one-group Student t -test; n = 3–4 each. The dashed line denotes 1.0 (no change over control). The brackets indicate groups that are significantly different by the Tukey subtest.

Article Snippet: Typically at confluency, NHBE cells in the 12-well plate (catalog no. 3512; Costar, Corning, NY) had a density of 300,000–500,000 cells/well in 2 mL of BEGM.

Techniques:

Journal: Environmental Health Perspectives

Article Title: Seasonal Variations in Air Pollution Particle-Induced Inflammatory Mediator Release and Oxidative Stress

doi: 10.1289/ehp.7996

Figure Lengend Snippet: Production of ROSs measured by DHR in ( A ) AM and ( B ) NHBE cells stimulated with coarse, fine, and ultrafine Chapel Hill pollution particles collected in four different months. Particles were added to NHBE cells at 11 μg/mL and to AMs at 50 μg/mL. In general, NHBE cells appeared more responsive to PM than were AMs because increases in DHR signals were similar between the two cell types even though NHBE cells were exposed to lower doses of PM. *Tukey adjusted p -value < 0.05; n = 3–4 each. The dashed line denotes 1.0 (no change over control). The brackets indicate groups that are significantly different by ANOVA and the Tukey subtest.

Article Snippet: Typically at confluency, NHBE cells in the 12-well plate (catalog no. 3512; Costar, Corning, NY) had a density of 300,000–500,000 cells/well in 2 mL of BEGM.

Techniques:

Journal: Environmental Health Perspectives

Article Title: Seasonal Variations in Air Pollution Particle-Induced Inflammatory Mediator Release and Oxidative Stress

doi: 10.1289/ehp.7996

Figure Lengend Snippet: Effects of PM from 12 different months on the release of ( A ) IL-6 in AMs and ( B ) IL-8 in NHBE cells. Particles were added to AMs at 50 μg/mL and to NHBE cells at 11 μg/mL. The data for December ultrafine PM were not available and were omitted in the figure; n = 3–4 each.

Article Snippet: Typically at confluency, NHBE cells in the 12-well plate (catalog no. 3512; Costar, Corning, NY) had a density of 300,000–500,000 cells/well in 2 mL of BEGM.

Techniques:

Journal: Environmental Health Perspectives

Article Title: Seasonal Variations in Air Pollution Particle-Induced Inflammatory Mediator Release and Oxidative Stress

doi: 10.1289/ehp.7996

Figure Lengend Snippet: Correlations between Cr and IL-8 release in NHBE cells incubated with ( A ) coarse, ( B ) fine, and ( C ) ultrafine PM from 12 months. The dashed line represents the linear regression line. The overall model p -values for coarse, fine, and ultrafine PM were 0.106, 0.003, and 0.036, respectively. R 2 = 0.6082 and 0.401 for fine and ultrafine PM, respectively.

Article Snippet: Typically at confluency, NHBE cells in the 12-well plate (catalog no. 3512; Costar, Corning, NY) had a density of 300,000–500,000 cells/well in 2 mL of BEGM.

Techniques: Incubation

Journal: Cell reports

Article Title: Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis

doi: 10.1016/j.celrep.2021.109347

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: NHBE-Bronchial Epi Cells w/RA, BEGM, cryo , Lonza , CC-2540.

Techniques: Recombinant, Lysis, TUNEL Assay, Positive Control, Negative Control, Staining, Transfection, Purification, SYBR Green Assay, Knock-Out, Microarray, Software